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In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism

Received: 23 November 2018     Accepted: 20 December 2018     Published: 15 February 2019
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Abstract

The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.

Published in American Journal of Biomedical and Life Sciences (Volume 7, Issue 1)
DOI 10.11648/j.ajbls.20190701.11
Page(s) 1-5
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Curcuma Longa, Staphylococcus Aureus, Staphylococcus Epidermidis, Streptococcus Salivarius, Extracts

References
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  • APA Style

    Mbah-Omeje, Kelechi Nkechinyere. (2019). In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. American Journal of Biomedical and Life Sciences, 7(1), 1-5. https://doi.org/10.11648/j.ajbls.20190701.11

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    ACS Style

    Mbah-Omeje; Kelechi Nkechinyere. In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. Am. J. Biomed. Life Sci. 2019, 7(1), 1-5. doi: 10.11648/j.ajbls.20190701.11

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    AMA Style

    Mbah-Omeje, Kelechi Nkechinyere. In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. Am J Biomed Life Sci. 2019;7(1):1-5. doi: 10.11648/j.ajbls.20190701.11

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  • @article{10.11648/j.ajbls.20190701.11,
      author = {Mbah-Omeje and Kelechi Nkechinyere},
      title = {In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {7},
      number = {1},
      pages = {1-5},
      doi = {10.11648/j.ajbls.20190701.11},
      url = {https://doi.org/10.11648/j.ajbls.20190701.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190701.11},
      abstract = {The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism
    AU  - Mbah-Omeje
    AU  - Kelechi Nkechinyere
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    N1  - https://doi.org/10.11648/j.ajbls.20190701.11
    DO  - 10.11648/j.ajbls.20190701.11
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 1
    EP  - 5
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20190701.11
    AB  - The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.
    VL  - 7
    IS  - 1
    ER  - 

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Author Information
  • Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Enugu State, Nigeria

  • Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Enugu State, Nigeria

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